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KMID : 1011420130180040541
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Journal of Korean Ophthalmic Optics Society
2013 Volume.18 No. 4 p.541 ~ p.548
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Transplantation of Marrow Stromal Cells into the Developing Mammal Retina
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Lee Eun-Shil
Kwon Oh-Ju
Ye Eun-Ah
Jeon Chang-Jin
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Abstract
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Purpose:Marrow stromal cells (MSCs) have been known for their potential to trans-differentiate into neural and
glial cells in vitro and in vivo. To investigate the influence of the developing host environment on the survival
and morphological and molecular differentiation, murine MSCs transplanted into the eye of Brazilian opossum
(Monodelphis domestica).
Methods:Enhanced green fluorescent protein (GFP) - expressing MSCs were transplanted
into developing Brazilian opossums. Animals were allowed to survive for up to 4 weeks after transplantation, at
which time the eyes were prepared for immunohistochemical analysis.
Results:Some transplanted MSCs
survived and showed morphological differentiation into neural cells with some processes within the host vitreous
chamber. Some transplanted cells expressed class III β-tubulin (TuJ1, a marker for neuronal cells) or glial
fibrillary acid protein (GFAP, a marker for glial cells) or Nestin (a marker for neural stem cells). In addition,
some transplanted cells were located in ganglion cell layer but did not show morphological and molecular
differentiation.
Conclusions:Our result show that the most effective stage of development for transplantation into
the retina was postnatal day 16, which retinas developmentally corresponded to postnatal day 4-5 days mouse
retina based on cell differentiation and lamination patterns. The present findings suggest that the age of the host
appears to play a key role in determining cell fate in vivo.
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KEYWORD
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Marrow stromal cells , Development , Retina , Transplantation , Differentiation , Migration
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